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Express Primer Tool for High Throughput Gene Cloning and Expression Poly Primer Design Tool Automated Cloning and Expression Methods Intranet Applications	Express Primer Tool for High Throughput Gene Cloning and Expression [ PROGRAM MANUAL AND DOWNLOAD ] Input File :    [descr] [instr] Optimal Tm : degrees Celcius. [descr] [instr] Maximum Tm Difference : degrees between forward and reverse primers. [descr] [instr] Tm Range : degrees from the Optimal Tm. [descr] [instr] Minimum Allowable Primer Length : nucleotides long. [descr] [instr] Maximum Allowable Primer Length : nucleotides long. [descr] [instr] (Optimal Tm and Tm range won't matter much if the option's optimized for individual primers) Options Omit sequences whose lengths are more than nucleotides long. yes : no [descr] [instr] Omit sequences whose lengths are less than nucleotides long. yes : no [descr] [instr] Optimize for individual primers. (choose "yes" for non-high-throughput) yes : no [descr] [instr] Force ATG start codon. yes : no [descr] [instr] Exclude start codons when generating forward primers. yes : no [descr] [instr] Exclude stop codons when generating reverse primers. yes : no [descr] [instr] Add extra C's or G's at the end of a primer if possible. yes : no [descr] [instr] Select extra sequence to be appended for forward primers* none SpeI XbaI AvrII [descr] [instr] Select extra sequence to be appended for reverse primers* none BglII BamHI [descr] [instr] * leave them empty if you don't want to append any sequences, thus don't want to check for any restriction sites
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